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JoVE Journal Neuroscience
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Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System

Article DOI: 10.3791/56436-v • 09:52 min • December 28th, 2017
December 28th, 2017 •
*1, *1, *1, 1, 1,2, 1, 1,3, 1, 1
1Department of Biochemistry and Molecular Biology and Molecular, Cellular and Integrated Neuroscience Program, Colorado State University, 2IBMC-Instituto de Biologia Molecular e Celular, i3S-Instituto de Investigaçãoe Inovação em Saúde, ICBAS, Universidade do Porto, 3Denali Therapeutics
* These authors contributed equally

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Summary
Automatic Translation

Presented here is a modified roller tube method for culturing and intermittent high-resolution imaging of rodent brain slices over many weeks with precise repositioning on photoetched coverslips. Neuronal viability and slice morphology are well maintained. Applications of this fully enclosed system using viruses for cell-type specific expression are provided.

Tags

Modified Roller Tube Method Brain Slice Culture Long-term Culture Enclosed System Repetitive Intermittent Imaging High-resolution Fluorescence Imaging Neuronal Development Neurodegenerative Disorders Synapse Formation Protein Expression Photo Etched Coverslips Viral Vectors Transgenes Cell Populations Microscope Imaging Pathological Changes Synapse Alteration Rodent Models Neurological Disorders
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