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JoVE Journal Developmental Biology
Analisi quantitativa intero-monta immunofluorescenza delle popolazioni di cellule progenitrici cardiache in embrioni di topo
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Analisi quantitativa intero-monta immunofluorescenza delle popolazioni di cellule progenitrici cardiache in embrioni di topo

Article DOI: 10.3791/56446-v • 09:42 min • October 12th, 2017
October 12th, 2017 •
1,2,3, 4, 5,6, 1,2,3
1Cell, Developmental, and Regenerative Biology Department, Icahn School of Medicine at Mount Sinai, 2Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, 3Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, 4Microscopy Core, Icahn School of Medicine at Mount Sinai, 5Department of Neuroscience, Icahn School of Medicine at Mount Sinai, 6Friedman Brain Institute, Icahn School of Medicine at Mount Sinai

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Qui, descriviamo un protocollo per immunofluorescenza intero supporto e basate su immagine quantitativa analisi volumetrica degli embrioni del mouse in fase precoce. Presentiamo questa tecnica come un potente approccio qualitativamente e quantitativamente valutare strutture cardiache durante lo sviluppo e proponiamo che possa essere ampiamente adattabile ad altri sistemi dell'organo.

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Biologia dello sviluppo problema 128 microscopia confocale analisi delle immagini imaging quantitativo organogenesi sviluppo del cuore embrione di topo
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