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JoVE Journal Developmental Biology
Análisis de la inmunofluorescencia de Whole-mount cuantitativa de las poblaciones progenitoras cardiacas en embriones de ratón
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Análisis de la inmunofluorescencia de Whole-mount cuantitativa de las poblaciones progenitoras cardiacas en embriones de ratón

Article DOI: 10.3791/56446-v • 09:42 min • October 12th, 2017
October 12th, 2017 •
1,2,3, 4, 5,6, 1,2,3
1Cell, Developmental, and Regenerative Biology Department, Icahn School of Medicine at Mount Sinai, 2Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, 3Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, 4Microscopy Core, Icahn School of Medicine at Mount Sinai, 5Department of Neuroscience, Icahn School of Medicine at Mount Sinai, 6Friedman Brain Institute, Icahn School of Medicine at Mount Sinai

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Aquí, describimos un protocolo para montaje entero de la inmunofluorescencia y análisis volumétrico cuantitativo basado en imágenes de embriones de ratón de etapa temprana. Presentamos esta técnica como un potente enfoque cualitativa y cuantitativamente evaluar estructuras cardiacas durante el desarrollo, y proponer que sea ampliamente adaptable a otros sistemas del órgano.

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Biología del desarrollo número 128 microscopia Confocal análisis de imagen imagen cuantitativa organogénesis desarrollo del corazón embrión de ratón
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