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Immunology and Infection

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JoVE Journal Immunology and Infection
Measuring Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells
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Measuring Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells

Article DOI: 10.3791/56448-v • 12:09 min • November 22nd, 2017
November 22nd, 2017 •
1,2, 1, 1, 1, 1
1Influenza Division, Centers for Disease Control and Prevention, 2Battelle

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Summary
Automatic Translation

Influenza neutralizing antibodies correlate with protection of influenza infections. Microneutralization assays measure neutralizing antibodies in human sera and are often used for influenza human serology. We describe a microneutralization assay using MDCK-SIAT1 cells to measure neutralizing antibody titers to contemporary 3C.2a and 3C.3a A(H3N2) viruses following influenza vaccination or infection.

Tags

Microneutralization Assay A(H3N2) Viruses MDCK-SIAT1 Cells Neutralizing Antibody Responses Influenza Immunity Vaccination Circulating H3N2 Viruses Antigenic Clusters Virus Propagation Virus Dilution Microtiter Plates
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