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Journal / Biochemistry / Diepe Proteoom profilering door isobaar Labeling, …
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Biochemistry

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JoVE Journal Biochemistry
Diepe Proteoom profilering door isobaar Labeling, uitgebreide vloeistofchromatografie, massaspectrometrie en Software-bijgewoonde kwantificering
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Diepe Proteoom profilering door isobaar Labeling, uitgebreide vloeistofchromatografie, massaspectrometrie en Software-bijgewoonde kwantificering

Article DOI: 10.3791/56474-v • 10:37 min • November 15th, 2017
November 15th, 2017 •
1, 1, 1, 2,3, 1, 1, 2, 1,2
1St. Jude Proteomics Facility, St. Jude Children's Research Hospital, 2Departments of Structural Biology and Developmental Neurobiology, St. Jude Children's Research Hospital, 3Heilongjiang University of Chinese Medicine

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We presenteren een protocol om te kwantificeren nauwkeurig eiwitten met isobaar etikettering, uitgebreide fractionering, bioinformatica tools en kwaliteitscontrole stappen in combinatie met vloeibare chromatografie geïnterfacet met een hoge resolutie massaspectrometer.

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Biochemie kwestie 129 bio-informatica interferentie isobaar labeling LC-MS/MS vloeibare chromatografie massaspectrometrie proteomics TMT
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