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Journal / Biochemistry / Dyb proteomet profilering af Isobar mærkning,…
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Biochemistry

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JoVE Journal Biochemistry
Dyb proteomet profilering af Isobar mærkning, omfattende væskekromatografi, massespektrometri og Software-assisteret kvantificering
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Dyb proteomet profilering af Isobar mærkning, omfattende væskekromatografi, massespektrometri og Software-assisteret kvantificering

Article DOI: 10.3791/56474-v • 10:37 min • November 15th, 2017
November 15th, 2017 •
1, 1, 1, 2,3, 1, 1, 2, 1,2
1St. Jude Proteomics Facility, St. Jude Children's Research Hospital, 2Departments of Structural Biology and Developmental Neurobiology, St. Jude Children's Research Hospital, 3Heilongjiang University of Chinese Medicine

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Vi præsenterer en protokol for at nøjagtigt kvantificere proteiner med Isobar mærkning, omfattende fraktionering, bioinformatik værktøjer og kvalitetskontrol trin i kombination med væskekromatografi interface til en høj opløsning massespektrometer.

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Biokemi sag 129 bioinformatik interferens Isobar mærkning LC-MS/MS liquid chromatography massespektrometri proteomics TMT
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