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Isolamento delle ghiandole mammarie Mouse intatto, intero per l'analisi di espressione di matrice extracellulare e la morfologia della ghiandola
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Isolamento delle ghiandole mammarie Mouse intatto, intero per l'analisi di espressione di matrice extracellulare e la morfologia della ghiandola

Article DOI: 10.3791/56512-v • 12:49 min • October 30th, 2017
October 30th, 2017 •
1, 2, 2
1Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, 2Department of Biomedical Sciences, GA-PCOM

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Qui, presentiamo un protocollo per l'isolamento del mouse intero, intatto ghiandole mammarie per studiare l'espressione di matrice extracellulare (ECM) e morfologia duttale. Mouse #4 ghiandole addominali sono state estratte da topi femmine nullipare 8-10 settimane vecchio, fissato in formalina neutra tamponata, sezionato e macchiato usando immunohistochemistry per proteine ECM.

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Problema 128 fisiologia immunohistochemistry matrice extracellulare ghiandole mammarie condotti
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