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Immunology and Infection

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JoVE Journal Immunology and Infection
An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection
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An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection

Article DOI: 10.3791/56515-v • 09:43 min • March 30th, 2018
March 30th, 2018 •
1, 2, 1, 3, 1, 2, 1
1Genetic Resources Center, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 2MOE Key Laboratory of Major Diseases in Children, National Key Discipline of Pediatrics, National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, 3State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences

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Summary
Automatic Translation

A simple method for obtaining NK and T cell clones from CAEBV patients was developed with high efficiency, a small amount of peripheral blood, and a low-dose of IL-2.

Tags

NK Cell Lines T Cell Lines Chronic Active Epstein-Barr Virus Infection Research Mechanism Of CAEBV Cell Proliferation Screening Inhibitors Peripheral Blood Iron Two Culture Cell Survival Human Serum Iron Two PBMC Gradient Centrifugation Cryo-preserved PBMC Trypan Blue
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