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Journal / Immunology and Infection / Mættede fedtsyrer fremkalde ceramid-associerede…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Mættede fedtsyrer fremkalde ceramid-associerede makrofag celledød
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A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry
Journal (Immunology and Infection)
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Mættede fedtsyrer fremkalde ceramid-associerede makrofag celledød

Article DOI: 10.3791/56535-v • 08:26 min • October 31st, 2017
October 31st, 2017 •
1, 1, 1, 1
1Department of Microbiology and Immunology, University of Louisville

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Vi illustrere en ligetil metode til at udlede murine primære makrofager fra knoglemarven cellerne og en enkel metode til at forberede BSA-fedtsyre konjugater. Så vi demonstrere at mættede fedtsyrer kan fremkalde makrofag celledød, og sådanne celledød er positivt associeret med cellulære ophobning af ceramid niveauer.

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Immunologi spørgsmålet 128 knoglemarv-afledte makrofager BSA (bovin serum albumin) mættet fedtsyre palmitinsyre stearinsyre ceramide celledød
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