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JoVE Journal Neuroscience
Visualisering af Thalamocortical Axon forgrening og Synapse dannelse i Organotypic Cocultures
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Visualisering af Thalamocortical Axon forgrening og Synapse dannelse i Organotypic Cocultures

Article DOI: 10.3791/56553-v • 06:16 min • March 28th, 2018
March 28th, 2018 •
1,2, 2
1Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University

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Denne protokol beskriver en metode til samtidige billeddannelse af thalamocortical axon forgrening og synapse dannelse i organotypic cocultures af thalamus og hjernebarken. Enkelte thalamocortical axoner og deres præsynaptiske terminaler er visualiseret ved en enkelt celle elektroporation teknik med DsRed og normal god landbrugspraksis-mærket synaptophysin.

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Neurovidenskab sag 133 Skive kultur hjernebarken thalamus synaptogenesis cytoskeletale arborization kortikal udvikling time-lapse imaging
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