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Organotypic Cocultures में Thalamocortical Axon बंटी और Synapse गठन का दृश्य
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Organotypic Cocultures में Thalamocortical Axon बंटी और Synapse गठन का दृश्य

Article DOI: 10.3791/56553-v • 06:16 min • March 28th, 2018
March 28th, 2018 •
1,2, 2
1Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University

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यह प्रोटोकॉल cocultures और सेरेब्रल thalamus के organotypic प्रांतस्था में thalamocortical axon शाखाओं और synapse गठन की एक साथ इमेजिंग के लिए एक विधि का वर्णन करता है । व्यक्तिगत thalamocortical axons और उनके presynaptic टर्मिनलों DsRed और GFP-टैग synaptophysin के साथ एक एकल सेल electroporation तकनीक द्वारा कल्पना कर रहे हैं ।

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तंत्रिका विज्ञान अंक 133 स्लाइस संस्कृति सेरेब्रल प्रांतस्था thalamus synaptogenesis axonal arborization cortical विकास समय चूक इमेजिंग
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