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Immunology and Infection

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JoVE Journal Immunology and Infection
Détection et suppression de nucléase Contamination durant la Purification de l’intégrase du Virus spumeux Prototype Recombinant
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Détection et suppression de nucléase Contamination durant la Purification de l’intégrase du Virus spumeux Prototype Recombinant

Article DOI: 10.3791/56605-v • 07:50 min • December 8th, 2017
December 8th, 2017 •
1, 1, 1, 1
1Department of Cancer Biology and Genetics, The Ohio State University College of Medicine

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Protéine de l’intégrase spumavirus prototype recombinante est souvent contaminée par une nucléase bactérienne pendant la purification. Cette méthode identifie la contamination nucléase et le supprime de la préparation finale de l’enzyme.

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Immunologie numéro 130 rétrovirus Prototype spumavirus intégrase nucléase purification des protéines
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