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Immunology and Infection

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JoVE Journal Immunology and Infection
Detección y eliminación de nucleasa contaminación durante la purificación de prototipo recombinante Virus Espumoso integrasa
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Detección y eliminación de nucleasa contaminación durante la purificación de prototipo recombinante Virus Espumoso integrasa

Article DOI: 10.3791/56605-v • 07:50 min • December 8th, 2017
December 8th, 2017 •
1, 1, 1, 1
1Department of Cancer Biology and Genetics, The Ohio State University College of Medicine

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Proteína de integrasa prototipo recombinante virus espumoso está a menudo contaminada con una nucleasa bacteriana durante la purificación. Este método identifica la contaminación nucleasa y lo quita de la preparación final de la enzima.

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Inmunología número 130 Retrovirus Virus espumoso de los prototipo integrasa nucleasa purificación de proteínas
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