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Journal / Bioengineering / Melhorado o hidrogel 3D culturas de células…
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Bioengineering

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JoVE Journal Bioengineering
Melhorado o hidrogel 3D culturas de células gliais primárias para In Vitro modelagem de Neuroinflammation
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Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator
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Melhorado o hidrogel 3D culturas de células gliais primárias para In Vitro modelagem de Neuroinflammation

Article DOI: 10.3791/56615-v • 09:19 min • December 8th, 2017
December 8th, 2017 •
1,2, 1,2, 1,3, 1,2,4, 3, 1,2,5
1Department of Psychiatry, University of Alberta, 2Alberta Innovates-Health Solutions Interdisciplinary Team in Smart Neural Prostheses (Project SMART), University of Alberta, 3Department of Chemical and Materials Engineering, University of Alberta, 4Division of Physical Medicine and Rehabilitation, University of Alberta, 5Centre for Neuroscience, University of Alberta

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Neste documento, apresentamos um protocolo para a cultura 3D do rato as células da glia derivado do cérebro, incluindo astrócitos, oligodendrócitos e micróglia. Vamos demonstrar a cultura de células primárias, síntese de hidrogel de ácido hialurônico methacrylated (HAMA), encapsulamento HAMAphoto-polimerização e celular e amostra de processamento de imagem microscópica confocal e varredura de elétrons.

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Bioengenharia edição 130 cultura de células 3D hidrogel glia primário astrocyte microglia oligodendrocyte neuroinflammation ácido hialurônico HAMA
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