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Journal / Bioengineering / Fabrikasjon av egendefinerte Agarose for celle…
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Bioengineering

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JoVE Journal Bioengineering
Fabrikasjon av egendefinerte Agarose for celle såing og vev Ring selvstendig montering med 3D-trykt muggsopp
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Fabrikasjon av egendefinerte Agarose for celle såing og vev Ring selvstendig montering med 3D-trykt muggsopp

Article DOI: 10.3791/56618-v • 08:16 min • April 2nd, 2018
April 2nd, 2018 •
1, 1, 1, 1,2, 1
1Biomedical Engineering, Worcester Polytechnic Institute, 2Gladstone Institute for Cardiovascular Disease

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Denne protokollen beskriver en plattform for fabrikere selv montert vev ringer i varierende størrelser ved hjelp av en tilpasset 3D-trykt plast mold. PDMS negativer er kurert i 3D-trykt mold; deretter er agarose støpt i herdet PDMS negativer. Celler er sådd i de resulterende agarose brønnene hvor de samlet i vev ringer.

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Bioteknologi problemet 134 3D-utskrift selv montert vev vascular tissue engineering agarose egendefinerte formen tissue engineering
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