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JoVE Journal Neuroscience
Aislamiento y cultivo de progenitores neuronales seguido por inmunoprecipitación de cromatina de histona 3 lisina 79 dimetilación marca
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Aislamiento y cultivo de progenitores neuronales seguido por inmunoprecipitación de cromatina de histona 3 lisina 79 dimetilación marca

Article DOI: 10.3791/56631-v • 10:09 min • January 26th, 2018
January 26th, 2018 •
1,2, 1, 1, 1, 1
1Institute for Anatomy and Cell Biology, Department of Molecular Embryology, Faculty of Medicine, University of Freiburg, 2Faculty of Biology, University of Freiburg

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Presentamos un método eficaz y reproducible para aislar las células progenitoras neurales de tejido cerebral embrionario y postnatal de inmunoprecipitación de cromatina (ChIP) de la histona 3 lisina 79 dimetilación (H3K79me2) - una marca de histona situada dentro de la cultura el dominio globular de la histona 3.

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Neurociencia número 131 cultivo de células cerebelosas cultivo de células corticales corteza desarrollo desarrollo cerebelosa metilación de H3K79 DOT1L viruta inhibición de la DOT1L
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