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JoVE Journal Neuroscience
En roman I Vitro Live bildebehandling analysen av astrocytter-mediert fagocytose bruker pH indikator-konjugerte Synaptosomes
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En roman I Vitro Live bildebehandling analysen av astrocytter-mediert fagocytose bruker pH indikator-konjugerte Synaptosomes

Article DOI: 10.3791/56647-v • 06:43 min • February 5th, 2018
February 5th, 2018 •
1, 1
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology

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Denne protokollen presenterer en i vitro live bildebehandling fagocytose analysen for å måle astrocyttene phagocytic kapasitet. Renset rotte astrocyttene og microglia brukes sammen med pH indikator-konjugerte synaptosomes. Denne metoden kan oppdage sanntid engulfment og fornedrelse kinetics og gir en passende screening plattform for å identifisere faktorer modulerende astrocytter fagocytose.

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Nevrovitenskap problemet 132 astrocytter microglia synaptosome pH indikator fagocytose engulfment degradering live bildebehandling
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