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Journal / Developmental Biology / IMMUNO florasan mikrotübüller ve Ex Vivo bağırsak …
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Developmental Biology

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JoVE Journal Developmental Biology
IMMUNO florasan mikrotübüller ve Ex Vivo bağırsak dokusu ve 3D Vitro bağırsak Organoids Centrosomal proteinler etiketleme
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IMMUNO florasan mikrotübüller ve Ex Vivo bağırsak dokusu ve 3D Vitro bağırsak Organoids Centrosomal proteinler etiketleme

Article DOI: 10.3791/56662-v • 09:51 min • December 13th, 2017
December 13th, 2017 •
1, 2, 1, 2, 1
1School of Biological Sciences, University of East Anglia, 2Norwich Medical School, University of East Anglia

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Biz iletişim kuralları izolasyon-in vivo dokusundan bağırsak 3D yapılar için mevcut ve organoids ve detay farklı fiksasyon ve protokolleri IMMUNO-etiketleme için Mikrotubul, en iyi duruma getirilmiş boyama vitro Bodrum matris gömülü, centrosomal ve bileske proteinler de gibi hücre kök hücre proteini Lgr5 de dahil olmak üzere işaretler.

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Gelişim biyolojisi sayı: 130 Organoid bağırsak sitoiskeleti mikrotübüller EB1 klip-170 ninein centrosome Lgr5 epiteli
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