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Investigando os prejudiciais efeitos de baixa pressão Plasma esterilização na sobrevivência de esporos de Bacillus subtilis usando Live celular microscopia

Article DOI: 10.3791/56666-v • 10:03 min • November 30th, 2017
November 30th, 2017 •
1, 1,2,3, 2, 4, 2, 4, 3, 1
1Department of Radiation Biology, Institute of Aerospace Medicine, Space Microbiology Research Group, German Aerospace Center (DLR e.V.), 2Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Ruhr-University Bochum, 3Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Biomedical Applications of Plasma Technology, Ruhr-University Bochum, 4Advanced Light and Electron Microscopy (ZBS 4), Robert Koch Institute

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Este protocolo ilustra as etapas consecutivas importantes, necessárias para avaliar a pertinência de monitoramento parâmetro de vitalidade e os processos de reparação do DNA em reviving esporos de Bacillus subtilis após o tratamento com plasma de baixa pressão de tracking proteínas através do tempo-resolvido microscopia confocal e microscopia eletrônica de reparo de DNA marcado com fluorescência.

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Biologia celular edição 129 esterilização Plasma descontaminação Bacillus subtilis esporos de resistência reparação de DNA células vivas de imagem SEM cLSM microscopia de fluorescência
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