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Journal / Genetics / Forbigående uttrykk av utenlandske gener i insekt …
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JoVE Journal Genetics
Forbigående uttrykk av utenlandske gener i insekt celler (sf9) for Protein funksjonelle analysen
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Forbigående uttrykk av utenlandske gener i insekt celler (sf9) for Protein funksjonelle analysen

Article DOI: 10.3791/56693-v • 11:12 min • February 22nd, 2018
February 22nd, 2018 •
1, 2, 2, 3, 1
1Department of Biotechnology and Animal Science, National Ilan University, 2Department of Agricultural Biology, College of Agriculture Life Sciences, Chonbuk National University, 3Department of Entomology, National Taiwan University

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Denne protokollen beskriver en varme sjokk-indusert protein uttrykk (pDHsp/V5-hans/sf9 celle system), som kan brukes for uttrykke utenlandske proteiner eller evaluere anti-apoptotisk aktiviteten til potensielle utenlandske proteiner og deres avkortet aminosyre syrer i insekt celler.

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Genetikk problemet 132 forbigående uttrykk protein funksjonelle analysen sf9 celle linje Lymantria xylina flere nucleopolyhedrovirus Ly-inhinbitor av apoptose-3 Drosophila varme sjokk 70 arrangøren pDHsp/V5-hans Drosophilia Reaper actinomycin-D
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