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Journal / Cancer Research / Ved hjælp af CRISPR/Cas9 gen redigering til at…
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Cancer Research

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JoVE Journal Cancer Research
Ved hjælp af CRISPR/Cas9 gen redigering til at undersøge de muterede aktivitet af Mutant Calreticulin i cytokin afhængige hæmatopoietisk celler
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Journal (Cancer Research)
Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis
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Journal (Cancer Research)
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Ved hjælp af CRISPR/Cas9 gen redigering til at undersøge de muterede aktivitet af Mutant Calreticulin i cytokin afhængige hæmatopoietisk celler

Article DOI: 10.3791/56726-v • 10:21 min • January 5th, 2018
January 5th, 2018 •
1, 1,2,3
1Division of Hematology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 2Broad Institute, 3Dana-Farber Cancer Institute, Harvard Medical School

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Målrettet gen redigering bruger CRISPR/Cas9 har høj grad lettet forståelsen af de biologiske funktioner af gener. Her, udnytte vi CRISPR/Cas9 metoden til model calreticulin mutationer i cytokin-afhængige hæmatopoietisk celler for at studere deres mutationer aktivitet.

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Kræftforskning sag 131 CRISPR Cas9 gen redigering sgRNA calreticulin sekventering reporter
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