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Cancer Research

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JoVE Journal Cancer Research
To-dimensionelle gelelektroforese kombineret med massespektrometri metoder til analyse af menneskelige hypofyseadenom væv proteom
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To-dimensionelle gelelektroforese kombineret med massespektrometri metoder til analyse af menneskelige hypofyseadenom væv proteom

Article DOI: 10.3791/56739-v • 12:34 min • April 2nd, 2018
April 2nd, 2018 •
1,2,3,4, 1,2,3, 1,2,3
1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, 2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital, Central South University, 3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital, Central South University, 4The State Key Laboratory of Medical Genetics, Central South University

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Her præsenterer vi en to-dimensionel gelelektroforese (2DE) kombineret med massespektrometri (MS) til at adskille og identificere menneskelige hypofyseadenom væv proteomet, som præsenterer en god og reproducerbare 2DE mønster. Mange proteiner er observeret i hver 2DE plet når analysere komplekse kræft proteomet med brug af høj-følsomhed MS.

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Kræftforskning spørgsmål 134 to-dimensionelle gelelektroforese isoelektrisk fokusering acrylamid gelelektroforese sølv farvning Coomassie farvning billedanalyse peptid masse fingeraftryk (PMF) Tandem massespektrometri (MS/MS) hypofyse Adenom proteom
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