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Een kinetische fluorescentie gebaseerde Ca2 + mobilisatie Assay te identificeren van G eiwit-gekoppelde Receptor agonisten en antagonisten Allosteric modulatoren

Article DOI: 10.3791/56780-v • 07:41 min • February 20th, 2018
February 20th, 2018 •
1, 1, 1, 1, 1
1Laboratory of Virology and Chemotherapy, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven

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De beschreven cellulaire assay is ontworpen voor de identificatie van CXC chemokine receptor 4 (CXCR4)-interagerende agenten die remmen of deze te stimuleren, concurrerend of allosterically, de intracellulaire Ca2 + release geïnitieerd door CXCR4 activering.

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Geneeskunde kwestie 132 G eiwit gekoppelde receptor chemokine CXC chemokine receptor 4 fluorescentie cel-gebaseerde assay Ca2 + mobilisatie agonist antagonist drugontdekking allosteric modulator kleine moleculen
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