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JoVE Journal Neuroscience
Kvantitativ måling af γ-Secretase-medieret Amyloid forløber Protein og hak kavalergang i celle-baserede Luciferase Reporter Assay platforme
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Kvantitativ måling af γ-Secretase-medieret Amyloid forløber Protein og hak kavalergang i celle-baserede Luciferase Reporter Assay platforme

Article DOI: 10.3791/56795-v • 06:40 min • January 25th, 2018
January 25th, 2018 •
*1, *1, *1, 1, 1, 1, 1
1Laboratory of Molecular Neurobiology, Institute of Cellular and Organismic Biology, Academia Sinica
* These authors contributed equally

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Vi har med succes skabt to substrat-specifikke γ-secretase assays. Begge cellebaserede assays præsenteres her er designet til at kvantificere γ-secretase enzymaktiviteter via output af firefly luciferase journalister.

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Neurovidenskab sag 131 Amyloid forløber protein Notch γ-Secretase Firefly luciferase ErbB2 Alzheimers sygdom Amyloid-β
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