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Journal / Neuroscience / Quantitative Messung der γ-Sekretase-vermittelten …
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JoVE Journal Neuroscience
Quantitative Messung der γ-Sekretase-vermittelten Amyloid-Precursor-Protein und Notch Spaltung in zellbasierten Luciferase Reporter Assay Plattformen
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Quantitative Messung der γ-Sekretase-vermittelten Amyloid-Precursor-Protein und Notch Spaltung in zellbasierten Luciferase Reporter Assay Plattformen

Article DOI: 10.3791/56795-v • 06:40 min • January 25th, 2018
January 25th, 2018 •
*1, *1, *1, 1, 1, 1, 1
1Laboratory of Molecular Neurobiology, Institute of Cellular and Organismic Biology, Academia Sinica
* These authors contributed equally

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Wir haben erfolgreich zwei Substrat-spezifische γ-Sekretase Assays generiert. Beiden zellbasierte Assays, die hier vorgestellten sollen γ-Sekretase enzymatischen Aktivitäten über den Ausgang des Firefly Luciferase Reporter zu quantifizieren.

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Neurowissenschaften Ausgabe 131 Amyloid Precursor Protein Kerbe γ-Sekretase Firefly Luciferase ErbB2 Alzheimer-Krankheit Amyloid-β
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