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Bioengineering

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JoVE Journal Bioengineering
At skabe et strukturelt realistisk Finite Element geometriske Model af en Cardiomyocyte til at studere rollen i cellulære arkitektur i Cardiomyocyte systembiologi
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At skabe et strukturelt realistisk Finite Element geometriske Model af en Cardiomyocyte til at studere rollen i cellulære arkitektur i Cardiomyocyte systembiologi

Article DOI: 10.3791/56817-v • 08:54 min • April 18th, 2018
April 18th, 2018 •
1,2,3, 2,3, 1,2,3, 2,4, 5, 6, 2,3,4,7,8, 9
1Cell Structure and Mechanobiology Group, University of Melbourne, 2Systems Biology Laboratory, Melbourne School of Engineering, University of Melbourne, 3Department of Biomedical Engineering, University of Melbourne, 4School of Mathematics and Statistics, Faculty of Science, University of Melbourne, 5Department of Engineering Science, University of Auckland, 6Advanced Microscopy Facility, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, 7ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, 8School of Medicine, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, 9Living Systems Institute, University of Exeter

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Denne protokol beskriver en ny metode for at skabe et rumligt detaljerede finite element model af den intracellulære arkitektur af cardiomyocytes fra elektronmikroskopi og konfokal mikroskopi billeder. Kraften i denne rumligt detaljerede model er påvist ved hjælp af casestudier i calcium signalering og bioenergetik.

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Bioteknologi spørgsmål 134 Cardiomyocyte hjertets ultrastruktur elektronmikroskopi Konfokal mikroskopi computerstøttet modellering finite element modellering systembiologi calcium signalering mitokondrier bioenergetik
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