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Guidede protokol for fækal mikrobielle karakterisering af 16S rRNA-amplikon sekventering
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Guidede protokol for fækal mikrobielle karakterisering af 16S rRNA-amplikon sekventering

Article DOI: 10.3791/56845-v • 08:05 min • March 19th, 2018
March 19th, 2018 •
1, 1, 1, 1, 1, 1, 2, 1,3, 1,4
1Sheba Medical Center, 2Children's Hospital of Pittsburgh of UPMC, 3Ariel University, 4Cincinnati Children's Hospital Medical Center

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Dette manuskript beskriver en detaljeret standardiseret protokol af høj overførselshastighed 16S rRNA-amplikon sekvensering. Protokollen introducerer en integreret, uniformerede, gennemførlige og billig protokol fra fecal prøvetagning gennem data analyser. Denne protokol giver mulighed for analyse af store mængder prøver med strenge standarder og flere kontrolelementer.

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Biologi sag 133 Microbiome 16S rRNA sekventering næste Generation Sequencing V4 variable region fækal prøver bibliotek forberedelse
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