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JoVE Journal
Biology

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定量实时反转录 PCR 技术对猕猴猴血浆 MicroRNA 水平的绝对量化
 
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定量实时反转录 PCR 技术对猕猴猴血浆 MicroRNA 水平的绝对量化

Article DOI: 10.3791/56850-v 10:23 min February 12th, 2018
February 12th, 2018

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Summary

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Click here for the English version.

本报告描述了一种测量血浆 miRNA 绝对水平的协议, 使用定量的实时反向转录 PCR 或不预放大。该议定书对血浆 miRNAs 的数量有了更好的了解, 并允许对来自不同研究或实验室的相应数据进行定性评估。

Tags

分子生物学 问题 132 microRNA 生物标志物 血浆 定量聚合酶链反应 (qPCR) 绝对量化 预扩
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