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Journal / Biochemistry / Un Western Blotting protocollo per un piccolo…
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JoVE Journal Biochemistry
Un Western Blotting protocollo per un piccolo numero di cellule staminali ematopoietiche
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Un Western Blotting protocollo per un piccolo numero di cellule staminali ematopoietiche

Article DOI: 10.3791/56855-v • 08:45 min • August 22nd, 2018
August 22nd, 2018 •
1,2,3, 1, 2
1Division of Experimental Hematology, Cincinnati Children's Hospital Medical Center, 2Department of Cell and Developmental Biology, Abramson Family Cancer Research Institute, Perelman School of Medicine, University of Pennsylvania, 3Department of Obstetrics and Gynecology, Southwest Hospital, Third Military Medical University

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Un standard Western blotting protocollo è stato ottimizzato per analizzare come pochi come 500 ematopoietiche staminali o cellule progenitrici. Ottimizzazione comporta un'attenta manipolazione del campione cellulare, limitando i trasferimenti tra tubi e Lisi direttamente le cellule in tampone di Laemmli.

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Biochimica problema 138 Western blotting cellule staminali ematopoietiche numeri di piccole cellule SDS-PAGE ridurre la sperimentazione animale del midollo osseo
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