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Journal / Biochemistry / Un Western Blot protocolo para una pequeña…
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Biochemistry

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JoVE Journal Biochemistry
Un Western Blot protocolo para una pequeña cantidad de células madre hematopoyéticas
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Un Western Blot protocolo para una pequeña cantidad de células madre hematopoyéticas

Article DOI: 10.3791/56855-v • 08:45 min • August 22nd, 2018
August 22nd, 2018 •
1,2,3, 1, 2
1Division of Experimental Hematology, Cincinnati Children's Hospital Medical Center, 2Department of Cell and Developmental Biology, Abramson Family Cancer Research Institute, Perelman School of Medicine, University of Pennsylvania, 3Department of Obstetrics and Gynecology, Southwest Hospital, Third Military Medical University

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Un estándar de Western Blot protocolo fue optimizado para el análisis de tan sólo 500 madre hematopoyética o células progenitoras. Optimización implica la cuidadosa manipulación de la muestra de células, limitando las transferencias entre los tubos y directamente lisis de las células en Tampón Laemmli.

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Bioquímica número 138 Western Blot las células madre hematopoyéticas un número pequeño de células SDS-PAGE reducir la experimentación con animales la médula ósea
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