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Bioengineering

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JoVE Journal Bioengineering
Högupplöst 3D Imaging av mänskliga bukspottkörteln Neuro-trångsynt nätverket
Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology
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Högupplöst 3D Imaging av mänskliga bukspottkörteln Neuro-trångsynt nätverket

Article DOI: 10.3791/56859-v • 09:54 min • January 29th, 2018
January 29th, 2018 •
1, 2, 3, 1, 1, 4, 5, 4, 1
1Department of Pathology, Immunology and Experimental Medicine, University of Florida, 2Heller School for Social Policy and Management, Brandeis University, 3Department of Medicine, College of Medicine, University of Florida, 4Department of Biomedical Engineering, College of Engineering, University of Florida, 5Department of Pediatrics, College of Medicine, University of Florida

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Här presenterar vi en protokoll bild mänskliga bukspottkörteln avsnitten i tre dimensioner (3D) med optimerad passiv clearing metoder. Detta manuskript visar dessa förfaranden för passiva optiska clearing följt av flera immunofluorescens färgning för att identifiera viktiga delar av de autonoma och sensoriska neuronnät innervating mänskliga holmar.

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Bioteknik fråga 131 bukspottkörteln holme pakten optisk clearing insulin glukagon β-celler immunofluorescens α-cellerna nerver Schwann celler Fredsgenomförande konfokalmikroskopi Lightsheet mikroskopi
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