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Journal / Biochemistry / Høj overførselshastighed, absolut bestemmelse af…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Høj overførselshastighed, absolut bestemmelse af indholdet af en markeret Protein i væv niveauer ved hjælp af kvantitative Dot duppes analyse (QDB)
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Høj overførselshastighed, absolut bestemmelse af indholdet af en markeret Protein i væv niveauer ved hjælp af kvantitative Dot duppes analyse (QDB)

Article DOI: 10.3791/56885-v • 08:10 min • August 21st, 2018
August 21st, 2018 •
*1, *2, 1, 3, 2, 1, 1,4, 2,3, 1
1Medicine and Pharmacy Research Center, Binzhou Medical University, 2Yantai Zestern Biotechnique Co. LTD, 3Precision Medicine research center, Binzhou Medical University, 4Department of Chemistry - BMC, Uppsala University
* These authors contributed equally

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Her viser vi en detaljeret proces af kvantitative dot skamplet analyse (QDB) ved at bestemme det absolutte indhold af en målrettet protein, udjævningen actin protein, gelsolin-lignende (CAPG), i tre forskellige mus væv. Vi demonstrere en høj produktivitet, praktisk, kvantitative immunblot teknik til biomarkør validering på niveauet cellulære og væv.

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Biokemi spørgsmålet 138 kvantitative Dot duppes analyse (QDB) immunblot høj produktivitet absolut bestemmelse Elisa Analysis.
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