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Biochemistry

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JoVE Journal Biochemistry
Usando tre colori singola molecola FRET per studiare la correlazione delle interazioni della proteina
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Usando tre colori singola molecola FRET per studiare la correlazione delle interazioni della proteina

Article DOI: 10.3791/56896-v • 11:22 min • January 30th, 2018
January 30th, 2018 •
1, 1, 1,2, 1
1Institute of Physical Chemistry, University of Freiburg, 2Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology

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Qui, presentiamo un protocollo per ottenere dati di tre colori smFRET e la sua analisi con un ensemble 3D modello di Markov nascosto. Con questo approccio, gli scienziati possono estrarre informazioni cinetiche da sistemi proteici complessi, tra cui il cooperativity o interazioni correlate.

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Problema 131 cinetica della proteina biofisica biochimica multi-colore smFRET TIRF HMM Hsp90 cooperatività singola molecola FRET
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