Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Bruker tre farger Single-molekylet bånd å studere …
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
Bruker tre farger Single-molekylet bånd å studere sammenhengen av Protein interaksjoner
Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation
Journal (Biochemistry)
Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation
Cryo-EM and Single-Particle Analysis with Scipion
Journal (Biochemistry)
Cryo-EM and Single-Particle Analysis with Scipion
 
Click here for the English version

Bruker tre farger Single-molekylet bånd å studere sammenhengen av Protein interaksjoner

Article DOI: 10.3791/56896-v • 11:22 min • January 30th, 2018
January 30th, 2018 •
1, 1, 1,2, 1
1Institute of Physical Chemistry, University of Freiburg, 2Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Her presenterer vi en protokoll for å få tre farger smFRET data og analyse med en 3D ensemble Hidden Markov Model. Med denne tilnærmingen, kan forskere trekke kinetic informasjon fra komplekse protein-systemer, inkludert cooperativity eller korrelert interaksjoner.

Tags

Biokjemi problemet 131 biofysikk protein kinetikk multi-farge smFRET TIRF HMM Hsp90 cooperativity enkelt-molekylet bånd
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter