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Journal / Neuroscience / Einfache Erstellung einer High-Yield-Kultur der…
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Neuroscience

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JoVE Journal Neuroscience
Einfache Erstellung einer High-Yield-Kultur der induzierten Neuronen aus Menschenhaut Erwachsenen Fibroblasten
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Einfache Erstellung einer High-Yield-Kultur der induzierten Neuronen aus Menschenhaut Erwachsenen Fibroblasten

Article DOI: 10.3791/56904-v • 09:07 min • February 5th, 2018
February 5th, 2018 •
1, 1, 1,2, 1, 1
1Department of Experimental Medical Science, Wallenberg Neuroscience Center, Division of Neurobiology and Lund Stem Cell Center, Lund University, 2John van Geest Centre for Brain Repair & Department of Neurology, Department of Clinical Neurosciences and Cambridge Stem Cell Institute, University of Cambridge

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Direkte neuronale Umprogrammierung erzeugt Neuronen, die im Alter von der somatischen Startzelle beibehalten. Hier beschreiben wir eine einzelne Vektor-basierte Methode um induzierte Neuronen von dermalen Fibroblasten von Erwachsenen menschlichen Spendern erhalten zu generieren.

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Neurowissenschaften Ausgabe 132 induzierte Neuronen Erwachsenen menschlichen dermalen Fibroblasten direkte neuralen Neuprogrammierung menschlichen Neuronen FACS-Reinigung Lentivirale Vektor
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