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JoVE Journal
Immunology and Infection

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X 射线辐照 Caco-2 细胞与 PBMC 间串扰的共培养方法研究
 
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X 射线辐照 Caco-2 细胞与 PBMC 间串扰的共培养方法研究

Article DOI: 10.3791/56908-v 11:40 min January 30th, 2018
January 30th, 2018

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Summary

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我们提出了一个协议来研究 X 射线辐照 Caco-2 和外周血单个核细胞 (PBMC) 之间的串扰。该协议始于 Caco-2 辐照和与 PBMC 共文化的建立;随后, 反式上皮电阻是定期测量超过48小时和西方印迹执行在 Caco-2 和 PBMC。

Tags

免疫学 问题 131 X 射线 电离辐射 Caco-2 大肠癌 跨上皮电阻 西部印迹 co 培养 外周血单个核细胞 紧密连接 免疫治疗 放疗
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