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Immunology and Infection

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JoVE Journal Immunology and Infection
Um método de co-cultura para investigar o Crosstalk entre raio-x irradiados PBMC e células Caco-2
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Um método de co-cultura para investigar o Crosstalk entre raio-x irradiados PBMC e células Caco-2

Article DOI: 10.3791/56908-v • 11:40 min • January 30th, 2018
January 30th, 2018 •
*1, *1, 1, 1, 2, 2, 2, 1
1Dipartimento di Fisica, Università degli Studi di Pavia, 2IRCCS Istituti Clinici Scientifici Maugeri
* These authors contributed equally

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Apresentamos um protocolo para investigar o crosstalk entre Caco-2 X-ray-irradiados e células mononucleares do sangue periférico (PBMC). O protocolo começa com a instalação e Caco-2 irradiação da cultura co com PBMC; posteriormente, resistência elétrica trans-epitelial é medida regularmente durante 48 h e ocidental do borrão realizados em PBMC e Caco-2.

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Imunologia questão 131 raios-x radiações ionizantes Caco-2 colorectal carcinoma resistência elétrica Trans-epitelial mancha ocidental co-cultura células mononucleares de sangue periférico junções apertadas imunoterapia radioterapia
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