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Journal / Biochemistry / Tvättmedel-fri ultrasnabb beredning av…
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Biochemistry

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JoVE Journal Biochemistry
Tvättmedel-fri ultrasnabb beredning av membranproteiner i Lipid lipidmonolager använder Fusogenic kompletterande laddade Proteoliposomes.
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Tvättmedel-fri ultrasnabb beredning av membranproteiner i Lipid lipidmonolager använder Fusogenic kompletterande laddade Proteoliposomes.

Article DOI: 10.3791/56909-v • 11:10 min • April 5th, 2018
April 5th, 2018 •
1, 2, 3, 2, 2
1First Pavlov State Medical University, 2Clarendon Laboratory, Department of Physics, Oxford University, 3Department of Biological Sciences, Southern Methodist University

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Här presenterar vi två ultrasnabb protokoll för beredning av membranproteiner in fusogenic proteoliposomes och fusion av sådan proteoliposomes med målet lipid lipidmonolager för tvättmedel-fri leverans av dessa membranproteiner in den postfusion lipidens. Kombinationen av dessa synsätt möjliggör snabb och lättkontrollerad montering av komplexa multi-komponent membransystem.

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Fråga 134 beredning membranproteiner biokemi proteoliposomes LUV GUV membran fusion elektron transportkedjan ATP-syntas ATP produktionen syntetisk biologi
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