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JoVE Journal
Biochemistry

This content is Open Access.

Udnytte en omfattende Immunoprecipitation berigelse System for at identificere en endogen posttranslationel modifikation profil for Target proteiner
 
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Udnytte en omfattende Immunoprecipitation berigelse System for at identificere en endogen posttranslationel modifikation profil for Target proteiner

Article DOI: 10.3791/56912-v 08:12 min January 8th, 2018
January 8th, 2018

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Summary

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Click here for the English version.

At undersøge flere, kan endogene posttranslationelle modifikationer for en target protein være ekstremt udfordrende. Teknikker beskrevet her udnytte en optimeret lysis buffer og filter system udviklet med specifikke PTM målretning, affinitet matricer til at registrere acetylation, ubiquitination, SUMOylation 2/3 og tyrosin fosforylering ændringerne for et mål protein ved hjælp af et enkelt, strømlinet system.

Tags

Biokemi sag 131 posttranslationel modifikation PTM afsløring Acetylation Ubiquitination fosforylering SUMOylation genomisk DNA fjernelse denaturering lysisbuffer immunoprecipitation PTM IP-protokol
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