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JoVE Journal Cancer Research
Detección del genoma-ancha de RNAi para identificar los Host factores que modulan la terapia de Virus oncolíticos
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Detección del genoma-ancha de RNAi para identificar los Host factores que modulan la terapia de Virus oncolíticos

Article DOI: 10.3791/56913-v • 08:51 min • April 3rd, 2018
April 3rd, 2018 •
1,2, 1,4, 1, 1, 1,2,3
1Children's Hospital of Eastern Ontario (CHEO) Research Institute, 2Department of Biology, Microbiology and Immunology, University of Ottawa, 3Department of Pediatrics, University of Ottawa, 4Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary

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Aquí se describe un protocolo para el empleo de proyección de ARNi de alto rendimiento para descubrir destinos host que se pueden manipular para mejorar la terapia de virus oncolíticos, específicamente rhabodvirus y vaccinia virus terapia, pero puede adaptarse fácilmente a otros oncolíticos plataformas de virus o para descubrir genes de host que modulan generalmente replicación del virus.

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Investigación de cáncer número 134 oncolíticos virus RNAi pantalla de alto rendimiento cáncer vaccinia rhabdovirus celular host genómica funcional
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