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Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
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Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

Article DOI: 10.3791/56945-v • 09:33 min • February 7th, 2018
February 7th, 2018 •
1, 2, 2,3
1Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, 2Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 3Oak Ridge Institute for Science and Education

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Summary
Automatic Translation

This manuscript describes the use of genetically-encoded fluorogenic reporters in an application of live-cell imaging for the examination of xenobiotic-induced oxidative stress. This experimental approach offers unparalleled spatiotemporal resolution, sensitivity, and specificity while avoiding many of the shortcomings of conventional methods used for the detection of toxicological oxidative stress.

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Imaging Approaches Assessments Toxicological Oxidative Stress Genetically-encoded Fluorogenic Sensors Redox Homeostasis Conventional Approaches Specificity Sensitivity Spacio-temporal Resolution Living Cells Confocal Microscope Laser Lines Environmental Controls Temperature Humidity Gas Concentration Experiment Duration Relative Humidity Objective Heater Heating Tape Condensation Prevention Microscope Components Sequential Excitation Emission
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