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JoVE Journal Cancer Research
Imaging benaderingen van evaluaties van toxicologische oxidatieve Stress met behulp van genetisch-gecodeerde Fluorogenic sensoren
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Imaging benaderingen van evaluaties van toxicologische oxidatieve Stress met behulp van genetisch-gecodeerde Fluorogenic sensoren

Article DOI: 10.3791/56945-v • 09:33 min • February 7th, 2018
February 7th, 2018 •
1, 2, 2,3
1Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, 2Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 3Oak Ridge Institute for Science and Education

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Dit manuscript beschrijft het gebruik van genetisch-gecodeerde fluorogenic verslaggevers in een toepassing voor live-cel imaging voor de behandeling van xenobioticum-geïnduceerde oxidatieve stress. Deze experimentele aanpak biedt ongeëvenaarde Spatio resolutie, gevoeligheid en specificiteit terwijl het vermijden van veel van de tekortkomingen van conventionele methoden voor de detectie van toxicologische oxidatieve stress.

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Kankeronderzoek kwestie 132 oxidatieve stress toxicologie fluorescentie Live-cel imaging glutathion waterstof peroxide
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