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JoVE Journal Cancer Research
Imaging tilnærminger til vurderinger av toksikologiske oksidativt Stress bruker genetisk kodet Fluorogenic sensorer
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Imaging tilnærminger til vurderinger av toksikologiske oksidativt Stress bruker genetisk kodet Fluorogenic sensorer

Article DOI: 10.3791/56945-v • 09:33 min • February 7th, 2018
February 7th, 2018 •
1, 2, 2,3
1Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, 2Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 3Oak Ridge Institute for Science and Education

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Dette manuskriptet beskriver bruk av genetisk kodet fluorogenic journalister i et program for live-celle imaging for undersøkelse av xenobiotic oval-indusert oksidativt stress. Denne eksperimentelle tilbyr enestående spatiotemporal oppløsning, sensitivitet og spesifisitet og unngå mange av svakhetene ved konvensjonelle metoder brukt for påvisning av toksikologiske oksidativt stress.

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Kreftforskning problemet 132 oksidativt stress toksikologi fluorescens Live-celle imaging glutation Hydrogen peroxide
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