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Microprobe kapillær elektroforese massespektrometri for encellede Metabolomics i Live Frog (Xenopus laevis) embryoner

Article DOI: 10.3791/56956-v • 12:16 min • December 22nd, 2017
December 22nd, 2017 •
1, 1, 2, 1,3
1Department of Chemistry, George Washington University, 2Department of Anatomy & Regenerative Biology, George Washington University, 3Department of Chemistry & Biochemistry, University of Maryland, College Park

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Vi beskrive de skridt, der gør det muligt hurtig in situ prøveudtagning af en lille del af en enkelt celle med høj præcision og minimal invasion ved hjælp af kapillar-baseret mikro-prøvetagning, for at lette kemiske karakterisering af et øjebliksbillede af metaboliske aktivitet i levende embryoner ved hjælp af en specialbygget enkelt celle kapillær elektroforese og massespektrometri platform.

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Kemi spørgsmålet 130 kapillær elektroforese electrospray Ionisation massespektrometri microsampling metabolomics enkelt celle Xenopus frog embryogenese
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