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Developmental Biology

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JoVE Journal Developmental Biology
Medición en tiempo real de la permeabilidad de la barrera epitelial en organoides Intestinal humano
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Medición en tiempo real de la permeabilidad de la barrera epitelial en organoides Intestinal humano

Article DOI: 10.3791/56960-v • 08:04 min • December 18th, 2017
December 18th, 2017 •
1, 1, 1, 1,2, 1,3
1Department of Internal Medicine, Division of Gastroenterology, University of Michigan, 2Department of Cell and Developmental Biology, University of Michigan, 3Department of Internal Medicine, Division of Infectious Disease, University of Michigan

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Este protocolo describe la medición de la permeabilidad de la barrera epitelial en el tratamiento farmacológico siguiente en tiempo real en humanos organitas intestinal mediante microscopía fluorescente y vivo microscopia celular.

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Biología del desarrollo número 130 organitas intestinal humano microinyección permeabilidad de la barrera epitelial enteroids cultivo de tejidos 3D proyección de imagen en vitro
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