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Genetics

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JoVE Journal Genetics
Effektiv produktion och identifiering av CRISPR/Cas9-genererade gen Knockouts i det modellsystem Danio rerio
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Journal (Genetics)
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression
Journal (Genetics)
The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression
 
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Effektiv produktion och identifiering av CRISPR/Cas9-genererade gen Knockouts i det modellsystem Danio rerio

Article DOI: 10.3791/56969-v • 11:27 min • August 28th, 2018
August 28th, 2018 •
1, 1, 1
1Department of Biochemistry, Purdue University

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Click here for the English version.

Riktade gen editering i systemet modell Danio rerio (Sebrafisken) har underlättats avsevärt genom uppkomsten av CRISPR-baserade metoder. Häri, beskriver vi ett strömlinjeformat, robust protokoll för generering och identifiering av CRISPR-derived nonsens alleler som innefattar heteroduplex rörlighet analysen och identifiering av mutationer med nästa generations sekvensering.

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Genetik fråga 138 Danio reriozebrafiskar CRISPR/Cas9 embryo Mikroskop gen knockout heteroduplex rörlighet assay nästa generations sekvensering omvänd genetik
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