Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Interactome: Domainome…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Interactome
Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers
Journal (Biology)
Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers
Sample Preparation and Fluorescence Measurement of Stained Transfected HEK293T Cells (Video) | JoVE
Journal (Biology)
Sample Preparation and Fluorescence Measurement of Stained Transfected HEK293T Cells (Video) | JoVE
 
Click here for the English version

Interactome: Domainome 图书馆建设、噬菌体展示和下一代测序的验证与选择协议

Article DOI: 10.3791/56981-v • 12:04 min • October 3rd, 2018
October 3rd, 2018 •
1, 2, 2, 3, 3, 3, 4, 1, 4, 5,6
1Department of Health Sciences, Università del Piemonte Orientale & IRCAD, Novara, Italy, 2Institute of Biomedical Technologies, National Research Council, Segrate, Milan, Italy, 3Institute of Biomedical Technologies, National Research Council, Bari, Italy, 4Department of Life Sciences, University of Trieste, Italy, 5Institute of Genetic and Biomedical Research, National Research Council, Rozzano, Milan, Italy, 6Humanitas Clinical and Research Center, Rozzano, Milan, Italy

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

所描述的协议允许构造、特征和选择 (针对选择的目标) 的 "domainome" 图书馆由任何 DNA 来源。这是通过一个结合不同技术的研究管道实现的: 噬菌体展示, 折叠记者和下一代测序, 以及一个用于数据分析的 web 工具。

Tags

生物学 140 期 噬菌体展示 下一代测序 Interactome 蛋白质领域 网络工具 折叠记者 蛋白质结构。
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter