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JoVE Journal Neuroscience
Single-cell Resolution fluorescens lever avbildning av Drosophila dygnsrytm klockor i Larval hjärnan kultur
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Single-cell Resolution fluorescens lever avbildning av Drosophila dygnsrytm klockor i Larval hjärnan kultur

Article DOI: 10.3791/57015-v • 07:05 min • January 19th, 2018
January 19th, 2018 •
1, 1
1Department of Genetics and Evolution, University of Geneva

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Målet med detta protokoll är att etablera ex vivo Drosophila larval hjärnan kultur optimerad för att övervaka dygnsrytm molekylär rytmer med långsiktiga fluorescens time-lapse imaging. Tillämpningen av denna metod på farmakologiska analyser diskuteras också.

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Neurovetenskap fråga 131 neurovetenskap dygnsrytm neuronal kommunikation time-lapse imaging ex vivo brain kultur fluorescerande reporter Drosophila
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