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Produktion og oprensning af Baculovirus for genterapi ansøgning
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Produktion og oprensning af Baculovirus for genterapi ansøgning

Article DOI: 10.3791/57019-v • 06:11 min • April 9th, 2018
April 9th, 2018 •
1,2, 1,2,3, 1,2
1Division of Experimental Hematology and Cancer Biology, Cincinnati Children's Hospital Medical Center, 2University of Cincinnati College of Medicine, 3Raymond G. Perelman Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia

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I denne protokol, er baculovirus produceret af forbigående Transfektion af baculovirus plasmid i Sf9 celler og forstærket i et serumfrit suspension kultur. Supernatanten er renset af heparin affinitet kromatografi og yderligere koncentreret af ultracentrifugering. Denne protokol er nyttigt for produktion og oprensning af baculovirus for gen terapi program.

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Genetik spørgsmål 134 Baculovirus Sf9 celler bacmid kromatografi rensning heparin kolonne ultracentrifuge.
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