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Journal / Developmental Biology / Combinational behandling av Trichostatin A og…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Combinational behandling av Trichostatin A og C-Vitamin forbedrer effektiviteten til kloning mus ved somatisk Cell Nuclear Transfer
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Combinational behandling av Trichostatin A og C-Vitamin forbedrer effektiviteten til kloning mus ved somatisk Cell Nuclear Transfer

Article DOI: 10.3791/57036-v • 09:52 min • April 26th, 2018
April 26th, 2018 •
1, 2, 3, 4, 1,5
1Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kindai University, 2Faculty of Biology-Oriented Science and Technology, Kindai University, 3Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, 4Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, 5Institute of Advanced Technology, Kindai University

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Vi beskriver en dramatisk forbedret metode for musen kloning trichostatin A, vitamin C og deionisert bovin serum albumin. Vi viser en forenklet, reproduserbare protokoll som støtter effektiv utvikling av klonet embryo. Denne metoden kan derfor bli en standardisert prosedyre for musen kloning.

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Utviklingspsykologi biologi problemet 134 somatisk Cell Nuclear Transfer Histone Deacetylase hemmere Trichostatin A Histone H3 lysin 9 Trimethylation Vitamin C deionisert bovin Serum Albumin mus Hemagglutinating Virus Japan konvolutten
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